1LGP
Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein complexed with tungstate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-23 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.210, 62.210, 54.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.000 - 2.000 |
R-factor | 0.232 * |
Rwork | 0.232 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.463 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.067 * | |
Total number of observations | 37545 * | |
Number of reflections | 8015 | |
Completeness [%] | 99.5 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 8000, Bis-Tris 6.5, DTT, Tungstate, VAPOR DIFFUSION, HANGING DROP, temperature 277.16K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | bis-Tris | 20 (mM) | pH6.5 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | reservoir | PEG8000 | 14-18 (%) | |
5 | 1 | reservoir | Bis-Tris | 100 (mM) | pH6.5 |
6 | 1 | reservoir | dithiothreitol | 5 (mM) |