1LAY
CRYSTAL STRUCTURE OF CYTOMEGALOVIRUS PROTEASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Detector technology | CCD |
Collection date | 1996-02-10 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 58.700, 58.700, 131.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.500 |
R-factor | 0.185 |
Rwork | 0.185 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.200 |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.060 |
Total number of observations | 22880 * |
Number of reflections | 7644 |
Completeness [%] | 90.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG400 | 30 (%) | |
2 | 1 | reservoir | phosphate citrate buffer | 0.1 (M) | |
3 | 1 | drop | protease | 3.5 (mg/ml) | |
4 | 1 | drop | PEG400 | 15 (%) | |
5 | 1 | drop | phosphate citrate buffer | 0.05 (M) |