1L8Q
CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9797,1.1272 |
Spacegroup name | I 2 3 |
Unit cell lengths | 155.752, 155.752, 155.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.700 |
R-factor | 0.23083 |
Rwork | 0.229 |
R-free | 0.25500 * |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.170 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.061 * | 0.415 * |
Number of reflections | 17366 | |
<I/σ(I)> | 15.5 | 5.1 |
Completeness [%] | 98.6 * | 98.9 * |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | sodium acetate, potassium chloride, hexanediol, PEG MME 2000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | TCEP | 1 (mM) | |
11 | 1 | reservoir | 4 (M) | ||
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | 400 (mM) | ||
4 | 1 | drop | 25 (mM) | ||
5 | 1 | drop | ADP | 5 (mM) | |
6 | 1 | drop | TCEP | 1 (mM) | |
7 | 1 | reservoir | 100 (mM) | pH5.0 | |
8 | 1 | reservoir | hexanediol | 14 (%) | |
9 | 1 | reservoir | PEG2000 MME | 15 (%) |