1L6P

N-terminal of DsbD (residues 20-144) from E. coli.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-07-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	53.206, 55.630, 102.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.139 *
Rwork	0.139
R-free	0.21500 *
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.137 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	1.650	1.650
Rmerge	0.074	0.382
Total number of observations	155538 *
Number of reflections	18609
<I/σ(I)>	28.2	5.11
Completeness [%]	98.5	96.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4 *	300	PEG 4000, sodium actetate, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1		0.5 (M)	pH7.4
3	1	1	Tris-HCl	0.1 (M)
4	1	2	sodium acetate	0.1 (M)	pH4.6
5	1	1	ammonium sulfate	0.2 (M)
6	1	1	PEG4000	25 (%)