1L5P
Crystal Structure of Trichomonas vaginalis Ferredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 1999-12-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.400, 64.550, 133.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 2.200 |
| Rwork | 0.248 |
| R-free | 0.31500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.136 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 * | |
| Number of reflections | 15472 | |
| Completeness [%] | 96.0 | 81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | used microseeding * |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19.0 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
