1L2P
ATP Synthase b Subunit Dimerization Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.99203 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 35.700, 40.760, 42.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 * - 1.550 |
| R-factor | 0.282 * |
| Rwork | 0.282 |
| R-free | 0.31300 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 13.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.027 | 0.117 |
| Number of reflections | 9455 * | |
| <I/σ(I)> | 25.1 | |
| Completeness [%] | 93.5 * | 83.1 * |
| Redundancy | 5.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 30% ISOPROPANOL, 45% 2-METHYL-2,4-PENTANEDIOL, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 18.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | 2-propanol | 45 (%) | |
| 3 | 1 | reservoir | MPD | 30 (%) |
