1L0Y
T cell receptor beta chain complexed with superantigen SpeA soaked with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.722, 83.734, 93.900 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 71.690 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.690 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.490 * |
| Rmerge | 0.107 | 0.481 |
| Total number of observations | 115588 * | |
| Number of reflections | 37614 * | |
| <I/σ(I)> | 10.3 | 1.4 |
| Completeness [%] | 96.7 * | 77.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG8K, MgCl, Tris, pH 8., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 15 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
