1L0N

native structure of bovine mitochondrial cytochrome bc1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	153.828, 153.828, 596.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.000 * - 2.600
R-factor	0.26143
Rwork	0.261
R-free	0.29700 *
RMSD bond length	0.020 *
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000 *
High resolution limit [Å]	2.600
Rmerge	0.061 *	0.520 *
Number of reflections	104476
Completeness [%]	95.2 *	96.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MOPS	50 (mM)	pH7.2
3	1	reservoir	ammonium acetate	20 (mM)
4	1	reservoir	glycerol	20 (%(w/v))
5	1	reservoir	decanoyl-N-mrthylglucamide	0.1 (%)