1KSX
Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-04 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.246, 103.650, 124.996 |
Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
Resolution | 50.000 * - 3.200 |
R-factor | 0.2634 * |
Rwork | 0.263 |
R-free | 0.28440 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f08 |
RMSD bond length | 0.007 |
RMSD bond angle | 21.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.195 | 0.584 |
Total number of observations | 125960 * | |
Number of reflections | 35038 | 3474 * |
<I/σ(I)> | 9.14 | 2.4 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | Ca(CF3COO)2, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ca(CF3COO)2 | ||
2 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 20 (mM) | ||
2 | 1 | reservoir | spermine tetrahydrochloride | 2.5 (mM) | |
3 | 1 | reservoir | ethylene glycol | 5 (%) |