1KSP
DNA polymerase I Klenow fragment (E.C.2.7.7.7) mutant/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 101.560, 101.560, 85.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.196 * |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | RIGID-BODY REFINEMENT |
| Starting model (for MR) | IN-HOUSE HIGH-RESOLUTION STRUCTURE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 * | 0.407 * |
| Total number of observations | 200444 * | |
| Number of reflections | 31495 * | |
| <I/σ(I)> | 12.1 | 2 |
| Completeness [%] | 96.3 | 96.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 18 * | Brick, P., (1983) J. Mol. Biol., 166, 453. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 1.1-1.2 (M) | |
| 2 | 1 | drop | PIPES | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | drop | protain | 10 (mg/ml) |
