1KR7
Crystal structure of the nerve tissue mini-hemoglobin from the nemertean worm Cerebratulus lacteus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.739, 1.740, 0.915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.721, 43.173, 60.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 1.500 |
| R-factor | 0.153 * |
| Rwork | 0.153 |
| R-free | 0.18702 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.239 |
| Total number of observations | 67606 * | |
| Number of reflections | 17906 | |
| <I/σ(I)> | 14.3 | 3.2 |
| Completeness [%] | 97.1 | 95.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | 4 * | ammonium sulfate, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.5 |
