1KOJ
Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 114 |
Detector technology | CCD |
Collection date | 2000-08-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.690, 115.970, 271.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.900 |
R-factor | 0.22 * |
Rwork | 0.220 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dqr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | 0.327 |
Total number of observations | 400188 * | |
Number of reflections | 96961 | 9158 * |
<I/σ(I)> | 23.1 | 4 |
Completeness [%] | 93.4 | 89 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | 14% PEG 8000, 250mM magnesium acetate, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 12-14 (%) | |
2 | 1 | reservoir | magnesium acetate | 250 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
4 | 1 | drop | protein | 15 (mg/ml) | |
5 | 1 | drop | 50 (mM) | ||
6 | 1 | drop | imidazole | 10 (mM) | pH7.5 |