1KOH

THE CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF A NOVEL RNA-BINDING DOMAIN FOUND IN THE HUMAN TAP NUCLEAR MRNA EXPORT FACTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	108
Detector technology	CCD
Collection date	2000-09-06
Detector	BRANDEIS - B4
Wavelength(s)	1.1000
Spacegroup name	P 41 21 2
Unit cell lengths	136.779, 136.779, 202.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.800
R-factor	0.275 *
Rwork	0.275
R-free	0.35500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ft8
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.940
High resolution limit [Å]	3.800	3.800
Rmerge	0.072	0.578
Number of reflections	18686
<I/σ(I)>	21.6	1.9
Completeness [%]	99.7 *	99.6
Redundancy	1.1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	20 *	sodium potassium tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	reservoir	potassium/sodium tartrate	1.1 (M)
3	1	reservoir	sodium citrate	0.05 (M)	pH5.6