1KFB
CRYSTAL STRUCTURE OF ALPHAT183V MUTANT OF TRYPTOPHAN SYNTHASE FROM SALMONELLA TYPHIMURIUM WITH Indole Glycerol Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8345 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.937, 60.255, 67.119 |
| Unit cell angles | 90.00, 94.84, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.21000 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 22.700 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 * | 0.262 * |
| Total number of observations | 153263 * | |
| Number of reflections | 54830 * | |
| Completeness [%] | 94.7 | 79.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 296 | PEG 8000, EDTA, spermine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | spermine | 2 (mM) | |
| 11 | 1 | reservoir | PEG8000 | 8-12 (%(w/v)) | |
| 2 | 1 | drop | Na+-bicine | 50 (mM) | pH7.8 |
| 3 | 1 | drop | Na+-EDTA | 10 (mM) | |
| 4 | 1 | drop | dithioerythritol | 1 (mM) | |
| 5 | 1 | drop | PLP | 20000 (nM) | |
| 6 | 1 | reservoir | Na+-bicine | 50 (mM) | pH7.8 |
| 7 | 1 | reservoir | dithioerythritol | 5 (mM) | |
| 8 | 1 | reservoir | Na+-EDTA | 5 (mM) | |
| 9 | 1 | reservoir | PLP | 0.1 (mM) |
