1KAX
70KD HEAT SHOCK COGNATE PROTEIN ATPASE DOMAIN, K71M MUTANT
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.900, 64.600, 46.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.700 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.26400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.033 |
| Total number of observations | 116095 * |
| Number of reflections | 38288 |
| Completeness [%] | 81.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | V8-venerated fragment | 8 (mg/ml) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | ADP | 1 (mM) | |
| 4 | 1 | drop | 1 (M) | ||
| 5 | 1 | reservoir | PEG8000 | 20 (%) |
