1K8R
Crystal structure of Ras-Bry2RBD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-08-06 |
| Detector | SIEMENS-NICOLET |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.030, 139.030, 112.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.236 * |
| Rwork | 0.235 |
| R-free | 0.31200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.375 * |
| Data reduction software | X-GEN |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.139 | 0.305 * |
| Total number of observations | 109981 * | |
| Number of reflections | 8526 | 788 * |
| <I/σ(I)> | 18.6 | 4.1 |
| Completeness [%] | 99.7 | 100 * |
| Redundancy | 12.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-25 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 20-25 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8. |
| 4 | 1 | reservoir | 0.2-0.4 (M) |
