1K6S
STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10 IN COMPLEX WITH A PHENYLBORONIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.245, 95.787, 125.074 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.200 - 2.030 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.600 | 2.140 |
| High resolution limit [Å] | 2.000 | 2.030 |
| Number of reflections | 37897 | |
| <I/σ(I)> | 7.2 | 3.7 |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | AMMONIUM SULFATE, CALCIUM CHLORIDE, BICINE PH 9; SOAKING AT PH 7 WITH HEPES, PEG400, 4-IODO-ACETAMIDO PHEYLBORONIC ACID, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
