1K3V
Porcine Parvovirus Capsid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 442.490, 443.050, 251.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.500 |
R-factor | 0.275 * |
Rwork | 0.286 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Feline Panleukopenia virus |
RMSD bond length | 0.010 |
RMSD bond angle | 26.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE (+ DM) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.290 | 0.574 |
Number of reflections | 254870 | |
<I/σ(I)> | 1.8 | 0.9 |
Completeness [%] | 41.3 | 28.6 |
Redundancy | 1.46 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 18 * | 20mM Hepes-NaOH 8mM CaCl, .05% sodium azide, 1-1.5% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 2 | HEPES-NaOH | 20 (mM) | pH6.5 |
3 | 1 | 2 | 8 (mM) | ||
4 | 1 | 2 | sodium azide | 0.05 (%(w/v)) | |
5 | 1 | 2 | PEG8000 | 1.0 | or 1.5% |