1JY5
RNase-related protein from Calystegia sepium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 62 |
| Unit cell lengths | 121.687, 121.687, 66.059 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.630 - 2.050 |
| R-factor | 0.2074 * |
| Rwork | 0.207 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dix |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.063 | 0.338 |
| Total number of observations | 163993 * | |
| Number of reflections | 35416 * | 1739 * |
| <I/σ(I)> | 20.71 | 5.12 |
| Completeness [%] | 85.5 | 66.3 * |
| Redundancy | 4.63 | 4.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | NaCl, 0.1M acetate buffer, MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.2 (M) | ||
| 2 | 1 | drop | acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | drop | MPD | 30 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
