1JUO
Crystal Structure of Calcium-free Human Sorcin: A Member of the Penta-EF-Hand Protein Family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 118.250, 118.250, 85.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.560 - 2.200 |
| R-factor | 0.212 * |
| Rwork | 0.218 |
| R-free | 0.25600 * |
| Structure solution method | MIR |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.199 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2000) |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 | 0.491 |
| Total number of observations | 167295 * | |
| Number of reflections | 28472 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 90.7 | 75.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium chloride, HEPES, EGTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sorcin | 0.004 (mM) | |
| 2 | 1 | drop | fluo-4 pentapossium salt | 0.010 (mM) | |
| 3 | 1 | drop | urea | 6.4 (mM) | or 7.8M |
| 4 | 1 | reservoir | 0.5 (M) | ||
| 5 | 1 | reservoir | HEPES | 0.1 | pH7.0 |
| 6 | 1 | reservoir | EGTA | 2.5 (mM) |
