1JOP
YHCH protein (HI0227)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0053, 1.0087 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.100, 152.300, 53.400 |
Unit cell angles | 90.00, 113.60, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.185 |
Rwork | 0.185 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.660 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.047 | 0.131 |
Number of reflections | 19314 | |
<I/σ(I)> | 19.9 | 6.2 |
Completeness [%] | 99.6 | 97.1 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Na acetate, PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |