1JMA
CRYSTAL STRUCTURE OF THE HERPES SIMPLEX VIRUS GLYCOPROTEIN D BOUND TO THE CELLULAR RECEPTOR HVEA/HVEM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9196 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 129.154, 129.154, 80.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.25900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.546 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 20.000 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.068 | 0.346 |
| Total number of observations | 272205 * | |
| Number of reflections | 24260 | |
| <I/σ(I)> | 11 | 5 |
| Completeness [%] | 99.7 | 99.9 * |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 291 | 1.8M Ammonium Sulfate and 5% Peg400 and Tris-HCL 100mM pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 2 | 1 | 1 | ammonium sulfate | 1.8 (M) | |
| 3 | 1 | 1 | PEG400 | 5 (%) |
