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1J7Z

Osmolyte Stabilization of Ribonuclease

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]293
Detector technologyIMAGE PLATE
Collection date1998-08-26
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameP 31 2 1
Unit cell lengths44.590, 44.590, 98.000
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution10.000 - 2.250
R-factor0.207

*

Rwork0.213
R-free0.27000

*

Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.200
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.851)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]10.0002.200
High resolution limit [Å]2.1002.100
Rmerge0.111
Completeness [%]100.0100
Redundancy53
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1unknown

*

4.75293Kim, E.E., (1992) Biochemistry, 31, 12304.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein12.5 (mg/ml)
21drop6.0 (M)
31dropsodium acetate0.1 (M)
41reservoirammonium sulfate80 (%sat)
51reservoirsodium acetate0.1 (M)

220113

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