1J3A
Crystal structure of ribosomal protein L13 from Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Detector technology | CCD |
Collection date | 2002-04-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.082, 51.542, 64.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
R-factor | 0.2094 |
Rwork | 0.209 |
R-free | 0.22750 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.170 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.700 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 17561 | |
Completeness [%] | 94.8 | 77.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.1M Phosphate-citrate, 20% PEG8000, 0.2M NaCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |