1J2W
Tetrameric Structure of aldolase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-25 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.280, 97.686, 137.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.290 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 * | 0.456 * |
| Total number of observations | 665297 * | |
| Number of reflections | 137192 * | |
| Completeness [%] | 98.5 * | 99.5 |
| Redundancy | 4.85 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.9 * | 295 | MPD, Tris HCl, magnesium chloride, pH 7.9, MICROBATCH, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 1 (M) | |
| 3 | 1 | 1 | sodium acetate | 1 (M) | |
| 4 | 1 | 1 | MPD | 30 (%) | pH4.9 |
