1J2J
Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.410, 61.894, 76.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.19988 |
| Rwork | 0.198 |
| R-free | 0.22700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1J2I |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.560 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.245 |
| Total number of observations | 194166 * | |
| Number of reflections | 30626 * | |
| <I/σ(I)> | 11 | 3 |
| Completeness [%] | 96.4 | 83.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | PEG3350, KI, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 10 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 5 | 1 | drop | protein | 30 (mg/ml) |
