1IPC
CRYSTAL STRUCTURE OF EUKARYOTIC INITIATION FACTOR 4E COMPLEXED WITH 7-METHYL GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Spacegroup name | P 43 |
Unit cell lengths | 88.190, 88.190, 38.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.192 * |
Rwork | 0.192 |
R-free | 0.22800 |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.280 * |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 * |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.079 * |
Number of reflections | 21848 * |
Completeness [%] | 89.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES-KOH | 100 (mM) | pH7.5 |
2 | 1 | reservoir | PEG6000 | 27 (%(w/v)) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) |