1IJ1
GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.683, 42.192, 42.682 |
Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.860 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.860 * | 1.860 |
Rmerge | 0.046 | 0.306 |
Total number of observations | 80296 * | |
Number of reflections | 8284 | |
<I/σ(I)> | 12.4 | 4.5 |
Completeness [%] | 97.6 | 94.4 * |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sparse matrix method * | 6 | 298 | 0.1 M Na Cacodylate, 20% PEG 8000, 0.2 M Zn Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium cacodylate | 0.1 (M) | |
2 | 1 | 1 | zinc acetate | 0.2 (M) | |
3 | 1 | 1 | PEG8000 | 20 (%) |