1IGO
Family 11 xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-08-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.190, 52.540, 65.410 |
| Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qh6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.309 |
| Total number of observations | 36059 * | |
| Number of reflections | 16265 | 1479 * |
| <I/σ(I)> | 7.67 | 2.38 |
| Completeness [%] | 95.0 | 87.1 |
| Redundancy | 2.22 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 295 | PEG 8000, sodium cacodylate, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | histidine | 25 (mM) | |
| 3 | 1 | drop | 3-N-morpholinopropanesulfonic acid | 30 (mM) | pH6.5 |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | PEG8000 | 30 (%) |
