1IFQ
Sec22b N-terminal domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92537, 0.97929, 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.244, 57.056, 99.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
Rwork | 0.231 |
R-free | 0.27400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.036 | 0.219 |
Number of reflections | 21210 | |
<I/σ(I)> | 31.2 | 5.3 |
Completeness [%] | 98.6 | 90.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 4 * | drop contains protein and reservoir solution in a 1:1 ratio * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG1000 | 27 (%) | |
3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
4 | 1 | reservoir | sodium citrate | 67 (mM) | |
5 | 1 | reservoir | BME | 20 (mM) | |
6 | 1 | reservoir | glycerol | 10 (%) |