1IES
TETRAGONAL CRYSTAL STRUCTURE OF NATIVE HORSE SPLEEN FERRITIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE D41A |
Synchrotron site | LURE |
Beamline | D41A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-06-01 |
Detector | MARRESEARCH |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 147.229, 147.229, 152.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.600 |
R-factor | 0.201 |
Rwork | 0.201 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hrs |
RMSD bond length | 0.012 |
RMSD bond angle | 2.400 |
Data reduction software | XDS |
Data scaling software | MARSCALE |
Phasing software | AMoRE |
Refinement software | X-PLOR (2.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.700 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.118 | 0.365 |
Total number of observations | 354156 * | |
Number of reflections | 51340 | |
<I/σ(I)> | 19.8 | 1.8 |
Completeness [%] | 89.5 | 60.8 |
Redundancy | 6.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium salfate | 0.66 (M) | |
2 | 1 | reservoir | cadmium sulfate | 80 (mM) | |
3 | 1 | reservoir | sodium azide | 3 (mM) |