1IC0
RED COPPER PROTEIN NITROSOCYANIN FROM NITROSOMONAS EUROPAEA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.41097,1.38035,1.37957,1.3495 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.903, 92.708, 97.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.100 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 * | 0.180 |
| Total number of observations | 570947 * | |
| Number of reflections | 41643 | |
| Completeness [%] | 98.1 | 99.6 * |
| Redundancy | 13.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | Hepes, Citrate, Jeffamine M-600, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 23K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | trisodium citrate | 1.4 (M) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | |
| 4 | 1 | reservoir | Jaffamine M-600 | 5 (%(v/v)) |
