1IBH
X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT M41I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 86.140, 86.140, 97.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 2.000 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.28700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | P.leiognathi Cu Zn SOD wild type |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.063 |
| Total number of observations | 106644 * |
| Number of reflections | 9449 |
| <I/σ(I)> | 26 |
| Completeness [%] | 99.6 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 | 100 * | PEG 8k, NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 30 (%) | |
| 2 | 1 | 1 | 0.1 (M) | ||
| 3 | 1 | 1 | sodium acetate | 0.05 (M) | |
| 4 | 1 | 1 | glycerol | 10 (%) |
