1I50
RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-04 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 122.700, 223.000, 376.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.28200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.058 | 0.344 |
| Number of reflections | 125251 | 12023 * |
| Completeness [%] | 99.0 | 96.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | Fu, J., (1999) Cell, 98, 799. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | dioxane | 50 (mM) | |
| 2 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | ammonium phosphate | 390 (mM) | or sodium phosphate, pH6.5 |
| 4 | 1 | reservoir | PEG6000 | ||
| 5 | 1 | drop | RNA | 5-8mg |
