1I4K
CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.842 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.397, 64.563, 129.862 |
Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.207 * |
Rwork | 0.207 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A seven membered ring of an Sm-like protein from Pyrococcus abyssii. |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.072 * | 0.353 * |
Total number of observations | 255898 * | |
Number of reflections | 63291 | |
<I/σ(I)> | 14.1 | 3.5 |
Completeness [%] | 99.3 | 99.9 |
Redundancy | 4.04 | 3.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 20 * | PEG 6000, sodium citrate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 12 (%) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.3 |