1I4G
Crystal structure of Staphylococcal enterotoxin A mutant H187A with reduced Zn2+ affinity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.660, 84.830, 86.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| Rwork | 0.178 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1esf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.600 * |
| Data reduction software | AUTOMAR |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.250 |
| Total number of observations | 158044 * | |
| Number of reflections | 30705 | |
| <I/σ(I)> | 14.2 | 1.6 |
| Completeness [%] | 95.2 | 92.7 |
| Redundancy | 5.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 298 | drop was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | tricine | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | PIPES | 100 (mM) |
