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1I4D

CRYSTAL STRUCTURE ANALYSIS OF RAC1-GDP COMPLEXED WITH ARFAPTIN (P21)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM14
Synchrotron siteESRF
BeamlineBM14
Temperature [K]100
Detector technologyCCD
Collection date2000-04-03
DetectorADSC QUANTUM 4
Wavelength(s)0.933
Spacegroup nameP 1 21 1
Unit cell lengths63.566, 47.462, 125.311
Unit cell angles90.00, 97.51, 90.00
Refinement procedure
Resolution20.000 - 2.500
Rwork0.238
R-free0.29800
Structure solution methodMAD / MR
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.590
High resolution limit [Å]2.5002.500
Rmerge0.1130.650
Number of reflections21742
<I/σ(I)>15.6
Completeness [%]94.2
Redundancy3.73
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

918

*

Tris, Peg20K, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21reservoirTris0.1 (M)
31reservoirPEG2000010 (%)

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