1I3Z
MURINE EAT2 SH2 DOMAIN IN COMPLEX WITH SLAM PHOSPHOPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.994, 59.500, 34.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.150 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAP SH2 |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.151 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.330 |
| Total number of observations | 24668 * | |
| Number of reflections | 7460 | |
| <I/σ(I)> | 16.6 | 2 |
| Completeness [%] | 97.0 | 75 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 22 * | 0.1 M sodium citrate, 30% PEG 8000, 10mM DTT, 25 mM ammonium sulphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 30 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 4 | 1 | reservoir | ammonium sulfate | 25 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
