1I2X
2.4 A STRUCTURE OF A-DUPLEX WITH BULGED ADENOSINE, SPERMIDINE FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-26 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.690, 47.710, 113.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.249 * |
| Rwork | 0.249 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 2.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.079 * |
| Number of reflections | 6180 * |
| Completeness [%] | 94.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 298 | spermidine, MPD. pH 6.0, VAPOR DIFFUSION, SITTING DROP at 298 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | spermidine | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.72 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 3 | 1 | reservoir | spermine | 25 (mM) | |
| 4 | 1 | reservoir | MPD | 40 (%) |
