1HWG

1:2 COMPLEX OF HUMAN GROWTH HORMONE WITH ITS SOLUBLE BINDING PROTEIN

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	145.610, 69.020, 76.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.199
Rwork	0.199
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2HHR
RMSD bond length	0.009
RMSD bond angle	1.530
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.700
High resolution limit [Å]	2.500	2.500
Rmerge	0.097 *	0.313 *
Number of reflections	25222
<I/σ(I)>	9	2.7
Completeness [%]	94.3	81.2
Redundancy	4.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.25 *	18 *	THE PROTEIN COMPLEX WAS CRYSTALLISED AT PH 6.5 USING 45% (W/V) LISO4 WITH 2% PEG-500 DME AND SOAKED TO PH 5.5 PRIOR TO DATA COLLECTION

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		46 (%(v/v)sat)
2	1	reservoir	PEG500	2 (%)
3	1	drop	protein	10 (mg/ml)