1HUJ
REFINED STRUCTURE OF YEAST INORGANIC PYROPHOSPHATASE AND ITS K61R MUTANT
Experimental procedure
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-26 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.050, 92.520, 51.580 |
Unit cell angles | 90.00, 99.47, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | LOW RESOLUTION STRUCTURE (PDB ENTRY 1PYP) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 31919 |
Completeness [%] | 91.6 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microliter drops | 6.5 | 277 | CRYSTALLIZATION CONDITIONS: 10 MG/ML PROTEIN IN 0.1 M MES (PH 6.5) BUFFER. 10 MICROLITER DROPS IN 9 WELL DEPRESSION PLATE IN A PLASTIC SANDWICH BOX CONTAINING 15 MILLILITER OF 0.1 M MES(PH 6.5) + 16 % MPD. EVERY ALTERNATE DAY, MPD CONCENTRATION INCREASED BY 1 % UNTIL FINAL CONCENTRATION REACHED 25 %. 4 DEGREE C. WITHIN 15 DAYS, 0.2 X 0.3 X 0.4 MM CRYSTALS., microliter drops, temperature 277K |