1HT0
HUMAN GAMMA-2 ALCOHOL DEHYDROGENSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-31 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.400, 71.500, 92.100 |
| Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1deh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.216 |
| Total number of observations | 188548 * | |
| Number of reflections | 47641 | |
| <I/σ(I)> | 21.3 | 6.7 |
| Completeness [%] | 97.9 | 86.8 |
| Redundancy | 4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 9 | 4 * | 100 mM Tris, 4 mM NAD+, 18% PEG 6000, 10 mg/ml protein, pH 9.0, VAPOR DIFFUSION, SITTING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG6000 | 18 (%) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | NAD+ | 4 (mM) | |
| 4 | 1 | 1 | protein | 10 (mg/ml) |
