1HQQ
MINIPROTEIN MP-2 (M9A) COMPLEX WITH STREPTAVIDIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-20 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.133, 89.299, 58.137 |
| Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
| Resolution | 20.840 - 1.700 |
| Rwork | 0.224 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Mp-2 streptavidin complex |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.840 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.302 |
| Number of reflections | 163318 | |
| <I/σ(I)> | 8.1 | 4.2 |
| Completeness [%] | 96.6 | 84.8 |
| Redundancy | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100 mM potassium acetate, ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
