1HMC

THREE-DIMENSIONAL STRUCTURE OF DIMERIC HUMAN RECOMBINANT MACROPHAGE COLONY STIMULATING FACTOR

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	33.370, 64.750, 157.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.197
Rwork	0.197
RMSD bond length	0.019
RMSD bond angle	3.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.070 *
Total number of observations	65871 *
Number of reflections	18842 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	PEG4000	23 (%)
4	1	drop	protein	10 (mg/ml)