1HL6

A novel mode of RBD-protein recognition in the Y14-mago complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	ADSC CCD
Spacegroup name	I 4
Unit cell lengths	140.177, 140.177, 68.579
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.224
Rwork	0.224
R-free	0.27200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.315
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.055 *	0.325
Number of reflections	23227
<I/σ(I)>	10.7	2.3
Completeness [%]	99.4	99.4
Redundancy	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5	4 *	SODIUM CITRATE PH5.25 PEG 3000 12-20%, pH 5.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	20 (%(w/v))
2	1	reservoir	sodium citrate	0.1 (M)	pH5.5