1HJS
Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Collection date | 2001-11-15 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.375, 136.145, 111.007 |
Unit cell angles | 90.00, 119.02, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.880* |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.20800 |
Structure solution method | OTHER |
RMSD bond length | 0.005 |
RMSD bond angle | 23.000 * |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.076 | 0.417 |
Number of reflections | 114360 | |
Completeness [%] | 97.6 | 89 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 30% PEG4000, 0.2 M AMMONIUM SULPHATE, 0.1M HEPES PH=7.5, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 30 (%) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |