1HJQ

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Collection date	2002-02-15
Spacegroup name	P 21 21 21
Unit cell lengths	45.254, 68.955, 135.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 2.550
R-factor	0.199
Rwork	0.199
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MTGAL (TRIS)
RMSD bond length	0.006
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.610
High resolution limit [Å]	2.550	2.550
Rmerge	0.140	0.440
Number of reflections	13628
Completeness [%]	94.6	90.8
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M SODIUM CACODYLATE PH=6.5 1.4 M SODIUM ACETATE TRIHYDRATE, pH 6.50