1H81
STRUCTURE OF POLYAMINE OXIDASE IN THE REDUCED STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 181.770, 181.770, 277.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b37 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.176 |
| Total number of observations | 1527497 * | |
| Number of reflections | 156868 | |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 20 * | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 4 (mg/ml) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | sodium phosphate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 42-50 (%sat) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) |
