1H53
Binding of Phosphate and Pyrophosphate ions at the active site of human Angiogenin as revealed by X-ray Crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 287 |
Collection date | 1998-09-15 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.383, 37.538, 38.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b1i |
RMSD bond length | 0.010 |
RMSD bond angle | 27.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.101 * | |
Total number of observations | 45669 * | |
Number of reflections | 8677 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 97.8 | 85.6 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.2 | 16 * | 20MM SODIUM CITRATE, 0.2M SODIUM POTASSIUM TARTARATE, 10% PEG 6000 PH 5.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium citrate | 20 (mM) | |
2 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
3 | 1 | reservoir | PEG6000 | 10 (%) |