1H3O
Crystal Structure of the Human TAF4-TAF12 (TAFII135-TAFII20) Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90730,0.97740 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.940, 36.825, 73.948 |
| Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.035 * | 0.122 * |
| Total number of observations | 61754 * | |
| Number of reflections | 25898 * | 2588 * |
| <I/σ(I)> | 19.9 | 4.45 |
| Completeness [%] | 98.9 * | 99.7 * |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.25 | 24 * | pH 5.25 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 5 (mM) | |
| 2 | 1 | drop | protein | 10-15 (mg/ml) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.25 |
| 4 | 1 | reservoir | ammonium sulfate | 2.2 (M) |
